Kendra Wilkinson starred as one of Hugh Hefner's three girlfriends when the show premiered in 2005. Fans were so invested in Wilkinson that she got her own spin-off show, Kendra, on E! after leaving the series in 2009.
After Kendra wrapped, Wilkinson landed another reality show on WE tv called Kendra On Top, which followed her journey as a mother of two and her marriage to her now ex-husband, Hank Baskett. Wilkinson also competed on a season of Dancing with the Stars in 2011.
We loved watching Bridget Marquardt on the E! series, from her investigating the mansion's possible haunted past to planning Hef's birthday, and the model went on to great success while on the show and after. She landed her own show called Bridget's Sexiest Beaches for the Travel Channel as well as appeared in cameo roles on shows and movies like Entourage, House Bunny and Curb Your Enthusiasm.
Now, Marquardt's interest in the paranormal has come full circle, as she now has a podcast called "Ghost Magnet" that interviews celebs who have experiences with the unexplainable. She also has found love with her long-time partner, Nicholas Carpenter.
Holly Madison had a successful career after her tenure at the Playboy Mansion, including competing on the show Dancing with the Stars before getting her own show Holly's World that centered around her career in Las Vegas, including her residency of the Peepshow burlesque show.
In 2015, she wrote her memoir about her time in the Playboy Mansion titled Down the Rabbit Hole: Curious Adventures and Cautionary Tales of a Former Playboy Bunny, following it up with a second memoir about her time in Vegas the next year.
She also became an advocate for PETA and has two children with her now ex-husband Pasquale Rotella, who she officially divorced last year.
While all of the ladies of The Girls Next Door had a special place in Hefner's heart, Crystal Harris famously got married to the Playboy magazine mogul in 2012.
The model, who was with Hefner until his death in 2017, now advocates for awareness around Lyme disease after announcing her diagnosis with the illness in 2016.
After the first generation of The Girls Next Door playmates left, twins Kristina and Karissa Shannon joined the Playboy Mansion in 2009 where we watched them celebrate their Playboy "summer issue" in Vegas, go backyard camping with Hef and even prepare for their small parts in Sofia Coppola's movie Somewhere.
After the show wrapped, the girls went on to join the ninth season of Celebrity Big Brother. The sisters also now co-own a salon, Glam Beverly Hills, in Los Angeles.